By train
From Florence to Rome: take a train to Terontola railways station and then, from Terontola, the coincidence to Assisi/Santa Maria degli Angeli. It takes approx. 45/60 minutes to reach Assisi from there.
From Ancona- Rome: take a train to Foligno and then another one to Perugia or Assisi -Santa Maria degli Angeli station. Assisi railway station is 3 km away from the historical centre of the city but you can get there easily by bus (approx.30 minutes).

By bus
From Santa Maria degli Angeli station/Assisi railway station: bus service departing from the square outside the station stop at Piazzale Matteotti (city centre).

By car
Motorway A14, Adriatic coastline: exit Cesena, take E45 to Città di Castello and then SS75 (Collestrada junction) to Assisi.
Motorway A1, from the North: exit Valdichiana, then take SS75 bis to Ponte San Giovanni and then Assisi.
Motorway A1, from the South:  exit Orte, then take the SS204 (direction: Terni) and E45 (or SS3bis) from Terni to Perugia. Once you get to Ponte San Giovanni, follow the signs to Assisi.